STI XXI - Preview - Surgical Overview
Evaluation of Biocompatibility of
Alloplastic Materials: Development of a Tissue Culture In Vitro Test System
Holger Gerullis, MD, Specialist in Urology, Consultant, Department of Urology, Lukas Hospital, Neuss, Germany, Oncologist, West German Cancer Center, University of Essen, Essen, Germany, Research Associate, German Centre for Assessment and Evaluation of Innovative Techniques in Medicine, Neuss, Germany, Evangelos Georgas, MD, Resident, Department of Urology, Lukas Hospital, Neuss, Germany, Christoph Eimer, MD, Specialist in Urology, Department of Urology, Lukas Hospital, Neuss, Germany, Peter E. Goretzki, MD, Professor, Head of Department , Department of General Surgery, Lukas Hospital, Neuss, Germany, Bernhard J. Lammers, MD, Head of Department of Coloproctology and Hernia Repair, Department of General Surgery, Lukas Hospital, Neuss, Germany, Bernd Klosterhalfen, MD, Professor, Head of Department, German Centre for Implant-Pathology, Duren, Germany, Mihaly Borós, MD, Professor, Head of Department , Department of Experimental Surgery, University of Szeged, Szeged, Hungary, Mohamed Wishahi, MD, Professor, Consultant, Department of Urology, Theodor Bilharz Research Institute, Cairo, Egypt, Gerd Heusch, MD, Professor, Head of Department, Institute for Pathophysiology, University of Essen, Essen, Germany, Thomas Otto, MD, Professor, Head of Department , Department of Urology, Lukas Hospital, Neuss, Germany, Oncologist, West German Cancer Center, University of Essen, Essen, Germany, Director, German Centre for Assessment and Evaluation of Innovative Techniques in Medicine, Neuss, Germany
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Abstract
- Optimized biocompatibility is a major requirement for alloplastic materials currently applied in surgical approaches for hernia, incontinence, and prolapse situations. Tissue ingrowth/adherence and formation of connective tissue seem to have important influence in mesh incorporation at the implant site. In an in vitro approach we randomly investigated 7 different mesh types currently used in surgeries with various indications with regard to their adherence performance. Using a tissue culture approach, meshes were incubated with tissue representative of fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. After 6 weeks, the meshes were assessed microscopically and a ranking of their adherence performance was established. Tissue culture was successful in 100% of the probes. We did not remark on interindividual differences concerning the growth and adherence performance after incubation with the different meshes in the investigated 10 patients. The ranking was consistent in all patients. In this test system, PVDF Dynamesh® (FEG Textiltechnik, Aachen, Germany) was the mesh with the best growth-in score. The test system was feasible and reproducible. Pore size seems to be a predictor of adherence performance. The test system may be a helpful tool for further investigations, and the predictive value should be assessed in further in vitro and in vivo experiments.
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